N-timer™ Insulin / Beta Cell Marker FACs

€ 375.00 – € 475.00

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SKU: NI001100UR Categories: ,

Product Description

N-timer™ Insulin (Protease Resistant)

The reagent stains live/viable β-cells in vitro or in vivo (WITHOUT FIXATION, WITHOUT PERMEABILIZATION). N-timer™ molecules are small synthetic peptides with a highly specific binding region targeting insulin. The N-timer™ Insulin molecule crosses tissues and cellular membranes in live islets, and remain only in cells containing high levels of Insulin. N-timer™ molecule is designed for use where proteases are present.  

N-timer™ Insulin is supplied with a reactive amine for conjugation with a dye of your choice and labeled variants.

The N-timer™ Insulin staining process is rapid, simple and reproducible. Each N-timer™ insulin carries one dye molecule and will transport the dye across biological membranes resulting in a simple and rapid staining process.

This reagent is recommended for working with β-cells that have been dispersed by the use of trypsin or other proteases or applications where proteases will be used after staining and the continued staining is desired.

Overview

Product Name

N-timer™ Insulin

Description

Synthetic peptide

Species Tested

Mouse and Human

Predicted to work with

Pig, Rat, Dog, Horse, Sheep, and Cow

Specificity

Targets the β-chain of Insulin

Tested applications

Fluorescence and Confocal Microscopy, Islet staining for Identification andflow cytometry (FACS)

Quantity

2 N-timer™ units (equivalent to 200μg of antibody)

Properties

Purity

≥ 95% single component

Form

Dry with DTT

Shipping Buffer

None. Reagent can be reconstituted in any buffer desired.

Storage Instructions

Store in the dark at +20⁰C short-term (4-5 weeks), store at +4⁰C for long term (up to 6 months) or aliquot and store at -20⁰C. Avoid repeated freeze thaw cycles

IMPORTANT

Select

, ,

Different Variants Available

THERE ARE VARIANTS OF THE N-TIMER™ AVAILABLE. PLEASE SELECT THE ONE YOU WOULD LIKE TO USE.
IMPORTANT NOTE

Reconstitution and conjugation of the N-timer™ The N-timer™ should be dissolved in 10 μl of the buffer recommended (by the dye manufacturer) for coupling the dye reagent. This N-timer™ is soluble in water, DMSO and DMF. It is recommended that 4μg of dye, ONLY USE AMINE REACTIVE DYE CHEMISTRY WITH THIS REAGENT, is used in the coupling reaction (according to the dye manufacturers instructions). It is also recommended that highly specific reagents for the attachment to amines are use as there are other free and vital nucleophiles are present (sulphhydral); thus chemistries like Isothiocynates are not recommended.After coupling to the dye, the N-timer™ can be purified by reverse phase HPLC. Do not use size separation products that are designed for proteins or anti bodies as the small size of the N-timer™will not separate from the dye and the loss of N-timer will be significant.

MSDS_ntimer  MSDS_ntimer

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