Product Description
N-timer™ Insulin (Protease Resistant)
|
The reagent stains live/viable β-cells in vitro or in vivo (WITHOUT FIXATION, WITHOUT PERMEABILIZATION). N-timer™ molecules are small synthetic peptides with a highly specific binding region targeting insulin. The N-timer™ Insulin molecule crosses tissues and cellular membranes in live islets, and remain only in cells containing high levels of Insulin. N-timer™ molecule is designed for use where proteases are present. N-timer™ Insulin is supplied with a reactive amine for conjugation with a dye of your choice and labeled variants. The N-timer™ Insulin staining process is rapid, simple and reproducible. Each N-timer™ insulin carries one dye molecule and will transport the dye across biological membranes resulting in a simple and rapid staining process. This reagent is recommended for working with β-cells that have been dispersed by the use of trypsin or other proteases or applications where proteases will be used after staining and the continued staining is desired. |
Overview
|
Product Name |
N-timer™ Insulin |
|
Description |
Synthetic peptide |
|
Species Tested |
Mouse and Human |
|
Predicted to work with |
Pig, Rat, Dog, Horse, Sheep, and Cow |
|
Specificity |
Targets the β-chain of Insulin |
|
Tested applications |
Fluorescence and Confocal Microscopy, Islet staining for Identification andflow cytometry (FACS) |
|
Quantity |
2 N-timer™ units (equivalent to 200μg of antibody) |
Properties
|
Purity |
≥ 95% single component |
|
Form |
Dry with DTT |
|
Shipping Buffer |
None. Reagent can be reconstituted in any buffer desired. |
|
Storage Instructions |
Store in the dark at +20⁰C short-term (4-5 weeks), store at +4⁰C for long term (up to 6 months) or aliquot and store at -20⁰C. Avoid repeated freeze thaw cycles |
MSDS_ntimer