This reagent allows the researcher to stain live/viable Sox 2 producing cells, both in vivo and in vitro (WITHOUT FIXATION, WITHOUT PERMEABILIZATION). N-timer™ molecules are small synthetic peptides with a highly specific binding region. The N-timer™ Sox 2 molecule has been designed to cross both tissues and cellular membrane barriers; and remain only in cells containing Sox 2 (in live cells/animals). N-timer™ Sox-2 is supplied with reactive amide for conjugation with a dye of your choice.Since the N-timer™ molecule carries the dye it is a complete reagent ready for use. The N-timer™ Sox 2 staining process is rapid, simple and reproducible. Each N-timer™ Sox 2 carries one dye molecule covalently coupled to the molecule and will transport the dye across biological membranes resulting in a simple and rapid staining process.
N-timer™ Sox 2 Transcriptional Marker
Predicted to work with
Targets near the C terminal portion of Sox 2
Flow Cytometry, Fluorescence microscopy, Confocal Microscopy, Live animal staining
2 N-timer™ units (equivalent to 200μg of antibody)
≥ 95% single component
Store at +20⁰C short-term (4-5 weeks) store at +4⁰C for long term (up to 1 year) or aliquot and store at -20⁰C. Avoid repeated freeze thaw cycles
0.1% sodium azide and 2mM DTT when made up to 200 µl.
Reconstitution of the N-timer
This pre-conjugated N-timer™ can be reconstituted in any buffer desired.
Reconstitution and conjugation of the N-timer™ The N-timer™ should be dissolved in 10 μl of the buffer recommended (by the dye manufacturer) for coupling the dye reagent. This N-timer™ is soluble in water, DMSO and DMF. It is recommended that 4μg of dye, ONLY USE AMINE REACTIVE DYE CHEMISTRY WITH THIS REAGENT, is used in the coupling reaction (according to the dye manufacturers instructions). It is also recommended that highly specific reagents for the attachment to amines are use as there are other free and vital nucleophiles are present (sulphhydral); thus chemistries like Isothiocynates are not recommended.After coupling to the dye, the N-timer™ can be purified by reverse phase HPLC. Do not use size separation products that are designed for proteins or anti bodies as the small size of the N-timer™will not separate from the dye and the loss of N-timer will be significant.